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國立陽明交通大學 應用化學系分子科學碩博士班 平松弘嗣所指導 余啟男的 利用532奈米激發超拉曼研究核苷酸和脫氧核醣核酸 (2021),提出Neon light png關鍵因素是什麼,來自於共振超拉曼光譜、核苷酸、去氧核醣核酸、共振效應、紫外共振拉曼。
而第二篇論文國立陽明交通大學 應用化學系碩博士班 陳月枝所指導 黃德一的 ⾮接觸式表⾯輔助常壓游離質譜法 (2021),提出因為有 質譜、大氣壓下游離質譜法、非接觸式大氣壓下游離法、常壓游離質譜法、表面輔助游離的重點而找出了 Neon light png的解答。
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利用532奈米激發超拉曼研究核苷酸和脫氧核醣核酸
為了解決Neon light png 的問題,作者余啟男 這樣論述:
去氧核糖核酸(DNA)作為生命的藍圖,儲存著生物體遺傳訊息,並透過轉錄及轉譯製造蛋白質,為了更了解DNA的功能我們需要從其分子結構著手,振動光譜像是紅外線吸收和拉曼光譜對DNA結構的研究持續至今,本文以超拉曼光譜作為第三種振動光譜學方法對其進行研究,由於超拉曼光譜有別於紅外線與拉曼光譜的選擇率,因此預期可以帶來新的結構訊息,此外DNA中的嘌呤與嘧啶在250到280奈米有紫外吸收,因為核鹼基的低結構對稱性,我們預期雙光子吸收也是可能的發生的。因此532奈米激發超拉曼符合雙光子共振條件,共振增強效應也使得我們能獲得高訊噪比的圖譜。本文的第一部分以DNA的組成單元核苷酸做為出發,測量四種核苷酸超拉
曼共振光譜,並將其與共振拉曼光譜比較,找出超拉曼光譜未來的潛力,於此同時我們也提供可能的振動模式,其有助之後應用於DNA。第二部分我們比較從核苷酸單體到形成長鏈二聚體時的光譜差異,探討氫鍵和構型改變帶來的影響,並提供鮭魚DNA超拉曼光譜中的各個峰對應的核苷酸來源。整體來說,我們成功的測量並分析了核苷酸與DNA超拉曼光譜,據我們所知這些超拉曼光譜為第一次的發表,本文結果也展示超拉曼光譜為DNA結構研究的一種新方法。
⾮接觸式表⾯輔助常壓游離質譜法
為了解決Neon light png 的問題,作者黃德一 這樣論述:
大氣壓化學游離質譜法和電噴灑游離質譜法可用於分析從低極性到高極性的分析物,因此能夠同時擁有兩種游離功能的游離源是相當理想的。本論文的主要目標是開發可用於分析具有不同極性和寬廣質量範圍的分析物之簡便質譜游離法。雖然現有大多數的游離方法需要利用到尖銳的導電金屬管/針做為游離噴灑器,但我們的研究發現平板和真實樣品即可直接用於輔助游離具有不同性質的分析物。在本論文的第一部分,我們證明利用鈦金屬平板或金鍍膜平板可用於輔助樣品游離,透過大氣壓化學游離法或電噴灑游離的過程對處於氣體、液體和固體形式的分析物進行游離。只需將樣品放在平板下方或平板上,可以直接將樣品中的分析物進行游離而由質譜分析。在平板上並沒有
額外施加任何電壓,因此此游離方法被稱為非接觸式常壓表面輔助游離法(ASAI)。鑑於使用這種 ASAI 方法可以檢測半揮發物或極性分析物,因此我們進一步證明了使用真實樣品如植物或藥丸做為游離載體的可行性。透過將真實樣品放置在質譜儀前,質譜儀可以很容易檢測到來自真實樣品中主要成分。此外,若將含有有機溶劑和去離子水的溶劑液滴滴在在真實樣品的表面上,也可以發生類似電噴灑游離的效果,可用於偵測樣品中極性較高的分析物,檢測來自真實樣品的主要成分。無需任何樣品前處理,質譜儀即可輕易分析到來自真實樣品的半揮發物或極性分析物。總而言之,我們已經成功開發了簡便的游離方法,可以通過使用平板做為游離載體,或直接使用真
實樣品來輔助游離其主要成分行質譜分析。本論文所開發的方法具有簡單和快速度等優點,因此,我們相信這些游離方法應該有潛力應用在高通量分析。
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