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在體外⾧期探討⾓膜塑型⽚與淚液蛋⽩之吸附關係

為了解決隱形眼鏡保養液7-11的問題，作者黃品萱 這樣論述：

摘要.......................................................................................................................................iABSTRACT .......................................................................................................................ii致謝.............................

........................................................................................................iv目錄.....................................................................................................................................v表目錄.......................................................

........................................................................viii圖目錄.................................................................................................................................x第一章緒論......................................................................................

................................11.1 前言.........................................................................................................................11.2 研究動機.................................................................................................................1第二章文獻探討..........

....................................................................................................22.1 近視.........................................................................................................................22.2 角膜塑型片材質............................................................

..........................................22.3 角膜塑型片鏡片設計..............................................................................................42.4 角膜塑型片矯正原理..............................................................................................52.5 淚膜...................................

......................................................................................52.5.1 淚膜中蛋白質..............................................................................................62.5.2 溶菌酶.................................................................................................

.........62.5.3 白蛋白..........................................................................................................72.5.4 淚膜中脂質..................................................................................................72.6 蛋白質與角膜塑型片吸附關係...............................................

......................72.7 多醣類..........................................................................82.7.1 海藻酸鈉......................................................................................................82.7.2 角叉菜膠........................................................................

..............................82.8 多醣類對蛋白質吸附影響..............................................................................8第三章關鍵問題與研究方法............................................................................103.1 關鍵問題.............................................................................

............................103.1.1 角膜塑型片的臨床問題.............................................................................103.2 研究方法........................................................................................................103.2.1 角膜塑型片對單一蛋白質的吸附量........................................

..................103.2.2 角膜塑型片針對溶菌酶吸附量..................................................................113.2.3 角膜塑型片針對白蛋白吸附量..................................................................113.2.4 角膜塑型片對膽固醇吸附量......................................................................113.2.5 保養液清洗後角膜塑型

片的光學性質......................................................11第四章實驗材料與方法....................................................................................124.1 實驗材料與藥品.............................................................................................124.2 人工淚液配置..........................

.......................................................................134.3 MT3保養液配置..............................................................................................154.4 蛋白質吸附實驗.............................................................................................164.4.1 目的.....

.......................................................................................................164.4.2 實驗儀器....................................................................................................164.4.3 分光光度測定法......................................................................

...................174.4.4 BCA測定法................................................................................................184.4.5 實驗條件....................................................................................................184.4.6 搓洗實驗流程.............................................

................................................184.4.7 實驗流程....................................................................................................194.4.8 蛋白質檢測方法.........................................................................................194.4.9 混和蛋白中溶菌酶濃度檢測方法..............

................................................204.4.10 混和蛋白中白蛋白濃度檢測方法............................................................224.4.11 推算角膜塑型片上蛋白質濃度................................................................244.4.12 膽固醇檢測方法...................................................................

....................244.5 推算角膜塑型片上膽固醇濃度....................................................................254.6 角膜塑型片性質檢測實驗...........................................................................264.6.1 目的...........................................................................................

.................264.6.2 實驗儀器與檢測方法.................................................................................264.7 實驗架構...............................................................................................................30第五章結果與分析......................................................

...............................................315.1 BTOX 189浸泡人工淚液後以保養液清洗吸附於角膜塑型片上蛋白質濃度.....315.2 Optimum Extra浸泡人工淚液後以保養液清洗吸附於角膜塑型片上蛋白質濃度....355.3 BTOX 189、Optimum Extra 溶菌酶當日吸附量比較.........................................405.4 BTOX 189、Optimum Extra光學性質及鏡片形貌........................................

.......405.5 複合多醣類於人工淚液對蛋白質的吸附影響.....................................................515.6 複合多醣類於人工淚液對膽固醇吸附影響.........................................................635.7 Poloxamer-407、Poloxamer-188清洗液對蛋白質吸附影響.................................685.8 複合多醣類於清洗保養液對蛋白質的吸附影響...............................

...................70第六章討論.................................................................................................................76第七章結論.................................................................................................................79 參考文獻.................................

.....................................................................................81

在體外探討隱形眼鏡保養液清洗對角膜塑型片與淚液蛋白、脂質之吸附與光學特性

為了解決隱形眼鏡保養液7-11的問題，作者曹苡菲 這樣論述：

過去幾年中，近視人口逐漸增加，目前控制近視方式包含眼鏡鏡片、軟式隱形眼鏡、藥劑(atropine and pirenzepine)以及角膜塑型片，其中角膜塑型片是一種夜間配戴治療透氣性鏡片，目的是在沒有隱形眼鏡或眼鏡的情況下，藉由改變角膜輪廓，減少患者白天屈光不正現象，以提供白天不戴眼鏡的清晰視野。夜間配戴淚液無法藉由眨眼來代謝，因此降低眼球表面抵抗力，讓淚液蛋白和脂質需吸附於角膜塑型片上，而角膜塑型片一旦清洗不乾淨，造成淚液蛋白和脂質吸附引起過敏反應與不適感，除了使配戴者感到不適之外，還會影響角膜塑型片原本光學特性，使鏡片規格和性質改變，影響視力矯正效果。為了探討角膜塑型片對淚液蛋白與脂質

吸附的效應，與隱形眼鏡保養液與淚液蛋白、脂質對於角膜塑型片光學性質的影響。本研究利用體外蛋白質定量測試法、酵素免疫分析法以及眼科光學儀器，量化淚液蛋白、脂質與隱形眼鏡保養液對角膜塑型片吸附與清潔能力以及保養液清潔後之光學特性，以了解清潔後角膜塑型片上淚液蛋白與脂質之吸附量與光學性質。