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在體外⾧期探討⾓膜塑型⽚與淚液蛋⽩之吸附關係

為了解決隱形眼鏡保養液的問題，作者黃品萱 這樣論述：

摘要.......................................................................................................................................iABSTRACT .......................................................................................................................ii致謝.............................

........................................................................................................iv目錄.....................................................................................................................................v表目錄.......................................................

........................................................................viii圖目錄.................................................................................................................................x第一章緒論......................................................................................

................................11.1 前言.........................................................................................................................11.2 研究動機.................................................................................................................1第二章文獻探討..........

....................................................................................................22.1 近視.........................................................................................................................22.2 角膜塑型片材質............................................................

..........................................22.3 角膜塑型片鏡片設計..............................................................................................42.4 角膜塑型片矯正原理..............................................................................................52.5 淚膜...................................

......................................................................................52.5.1 淚膜中蛋白質..............................................................................................62.5.2 溶菌酶.................................................................................................

.........62.5.3 白蛋白..........................................................................................................72.5.4 淚膜中脂質..................................................................................................72.6 蛋白質與角膜塑型片吸附關係...............................................

......................72.7 多醣類..........................................................................82.7.1 海藻酸鈉......................................................................................................82.7.2 角叉菜膠........................................................................

..............................82.8 多醣類對蛋白質吸附影響..............................................................................8第三章關鍵問題與研究方法............................................................................103.1 關鍵問題.............................................................................

............................103.1.1 角膜塑型片的臨床問題.............................................................................103.2 研究方法........................................................................................................103.2.1 角膜塑型片對單一蛋白質的吸附量........................................

..................103.2.2 角膜塑型片針對溶菌酶吸附量..................................................................113.2.3 角膜塑型片針對白蛋白吸附量..................................................................113.2.4 角膜塑型片對膽固醇吸附量......................................................................113.2.5 保養液清洗後角膜塑型

片的光學性質......................................................11第四章實驗材料與方法....................................................................................124.1 實驗材料與藥品.............................................................................................124.2 人工淚液配置..........................

.......................................................................134.3 MT3保養液配置..............................................................................................154.4 蛋白質吸附實驗.............................................................................................164.4.1 目的.....

.......................................................................................................164.4.2 實驗儀器....................................................................................................164.4.3 分光光度測定法......................................................................

...................174.4.4 BCA測定法................................................................................................184.4.5 實驗條件....................................................................................................184.4.6 搓洗實驗流程.............................................

................................................184.4.7 實驗流程....................................................................................................194.4.8 蛋白質檢測方法.........................................................................................194.4.9 混和蛋白中溶菌酶濃度檢測方法..............

................................................204.4.10 混和蛋白中白蛋白濃度檢測方法............................................................224.4.11 推算角膜塑型片上蛋白質濃度................................................................244.4.12 膽固醇檢測方法...................................................................

....................244.5 推算角膜塑型片上膽固醇濃度....................................................................254.6 角膜塑型片性質檢測實驗...........................................................................264.6.1 目的...........................................................................................

.................264.6.2 實驗儀器與檢測方法.................................................................................264.7 實驗架構...............................................................................................................30第五章結果與分析......................................................

...............................................315.1 BTOX 189浸泡人工淚液後以保養液清洗吸附於角膜塑型片上蛋白質濃度.....315.2 Optimum Extra浸泡人工淚液後以保養液清洗吸附於角膜塑型片上蛋白質濃度....355.3 BTOX 189、Optimum Extra 溶菌酶當日吸附量比較.........................................405.4 BTOX 189、Optimum Extra光學性質及鏡片形貌........................................

.......405.5 複合多醣類於人工淚液對蛋白質的吸附影響.....................................................515.6 複合多醣類於人工淚液對膽固醇吸附影響.........................................................635.7 Poloxamer-407、Poloxamer-188清洗液對蛋白質吸附影響.................................685.8 複合多醣類於清洗保養液對蛋白質的吸附影響...............................

...................70第六章討論.................................................................................................................76第七章結論.................................................................................................................79 參考文獻.................................

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